035

Title:

CikA Modulates the Effect of KaiA on the Period of the Circadian Oscillation in KaiC Phosphorylation

Discipline: Chemistry

Presenter:

Manpreet Kaur

Abstract:

Cyanobacteria contain a circadian oscillator that can be reconstituted in vitro. In the reconstituted circadian oscillator, the phosphorylation state of KaiC oscillates with a circadian period, spending about 12 h in the phosphorylation phase and another 12 h in the dephosphorylation phase. Although some entrainment studies have been performed using the reconstituted oscillator, they were insufficient to fully explain entrainment mechanisms of the cyanobacterial circadian clock due to the lack of input pathway components in the in vitro oscillator reaction mixture. Here, we investigate how an input pathway component, CikA, affects the phosphorylation state of KaiC in vitro. In general, CikA affects the amplitude and period of the circadian oscillation of KaiC phosphorylation by competing with KaiA for the same binding site on KaiB. In the presence of CikA, KaiC switches from its dephosphorylation phase to its phosphorylation phase prematurely, due to an early release of KaiA from KaiB as a result of competitive binding between CikA and KaiA. This causes hyperphosphorylation of KaiC and lowers the amplitude of the circadian oscillation. The period of the KaiC phosphorylation oscillation is shortened by adding increased amounts of CikA. A constant period can be maintained as CikA is increased by proportionally decreasing the amount of KaiA. Our findings give insight into how to reconstitute the cyanobacterial circadian clock in vitro by the addition of an input pathway component, and explain how this affects circadian oscillations by directly interacting with the oscillator components.

Keywords: CikA, cyanobacteria, Circadian clock, input pathway, in vitro

Author(s):

Manpreet Kaur, Amy Ng, Pyonghwa Kim, Casey Diekman, and Yong-Ick Kim

Funding Acknowledgements:

New Jersey Institute of Technology start-up grant to Y.I. Kim, and theNational Science Foundation grant DMS-1555237 and the U.S. Army Research Office grant W911NF-16-1-0584 to C. Diekman